CPSA Shanghai 2012
From Bench to Decision Making - From Basics to Application
April 25 - 27, 2012
Renaissance Shanghai Pudong Hotel
Shanghai, China
Abstract
Wednesday
Lunch and Learn Workshop
Use of Cell-based Assays for in vitro Drug Metabolism and Drug Transport Applications
Thursday, April 26th
12:00 pm - 1:00 pm
Instructor:
Na Li, Ph.D., Staff Scientist, BD Biosciences – Discovery Labware
Primary human hepatocytes are often considered the "Gold Standard" for drug ADME studies. Hepatocytes freshly isolated from human donor issues contain the full complement of phase I and phase II hepatic enzymes, including all co-factors needed for drug metabolism, and express both drug uptake transporters (SLC protein family), e.g. NTCP, OATP1B1/1B3, and OCT1, and efflux transporters (ABC protein family) e.g. MRP2, BCRP, P-gp and BSEP. Upon cultured in sandwich configuration with Collagen I and BD Matrigel™, primary hepatocytes are re-polarized and form an in vivo-like bile canaliculi network that facilitates the assessment of efflux and uptake drug transporter activities. The expanding use of hepatocyte model for drug metabolism and drug transporter application continues to enhance the predictive capabilities of in vitro investigations. The first part of this presentation will focus on the application of platable cryopreserved human hepatocytes for enzyme induction and metabolic stability assays for slowly metabolized compounds. In the second part, the seminar will focus on illustrating cell based assays to characterize SLC transporter mediated drug uptake and ABC transporter mediated efflux using both plated and suspension hepatocytes.
Brief C.V. of Dr Na Li
Dr. Na Li has been a Staff Scientist with BD Biosciences for over 3 years. Her expertise spans the areas of in vitro drug transporter models and application in drug ADME, translational aspect of drug transporter and in vitro to in vivo correlations (IVIVC) of transporter mediated elimination. Prior to joining BD Biosciences, Dr. Li worked as postdoctoral fellow at Pfizer St. Louis Research Laboratories with Pharmacokinetics, Dynamics & Metabolism (PDM) organization, where her major research focus is on drug transporters proteomics and its application in IVIVC. Dr. Na Li received her B.S degree in Biology from Fudan University, Shanghai, China, and her Ph.D. in Pharmacology from Dartmouth Medical School, Hanover, NH. She has published 13 peer-reviewed articles in the area of drug transporter.
